Department
Biology
Document Type
Article
Publication Date
6-2012
Abstract
We generated a high-quality reference genome sequence for foxtail millet (Setaria italica). The ~400-Mb assembly covers ~80% of the genome and >95% of the gene space. The assembly was anchored to a 992-locus genetic map and was annotated by comparison with >1.3 million expressed sequence tag reads. We produced more than 580 million RNA-Seq reads to facilitate expression analyses. We also sequenced Setaria viridis, the ancestral wild relative of S. italica, and identified regions of differential single-nucleotide polymorphism density, distribution of transposable elements, small RNA content, chromosomal rearrangement and segregation distortion. The genus Setaria includes natural and cultivated species that demonstrate a wide capacity for adaptation. The genetic basis of this adaptation was investigated by comparing five sequenced grass genomes. We also used the diploid Setaria genome to evaluate the ongoing genome assembly of a related polyploid, switchgrass (Panicum virgatum).
Recommended Citation
Bennetzen, Jeffrey L. and Triplett, Jimmy K., "Reference Genome Sequence of the Model Plant Setaria" (2012). Research, Publications & Creative Work. 63.
https://digitalcommons.jsu.edu/fac_res/63
Publication/Presentation Information
Bennetzen, J.L. et al. (2012) Reference genome sequence of the model plant Setaria. Nature Biotechnology, 30(6): 555-564. doi:10.1038/nbt.2196