Department
Biology
Document Type
Article
Publication Date
2024
Abstract
Biomedicine today is experiencing a shift towards decentralized data collection, which promises enhanced reproducibility and collaboration across diverse laboratory environments. This inter-laboratory study evaluates the performance of biocytometry, a method utilizing engineered bioparticles for enumerating cells based on their surface antigen patterns. In centralized and aggregated inter-lab studies, biocytometry demonstrated significant statistical power in discriminating numbers of target cells at varying concentrations as low as 1 cell per 100,000 background cells. User skill levels varied from expert to beginner capturing a range of proficiencies. Measurement was performed in a decentralized environment without any instrument cross-calibration or advanced user training outside of a basic instruction manual. The results affirm biocytometry to be a viable solution for immunophenotyping applications demanding sensitivity as well as scalability and reproducibility and paves the way for decentralized analysis of rare cells in heterogeneous samples.
Recommended Citation
Fikar, P., Alvarez, L., Berne, L., Cienciala, M., Kan, C., Kasl, H., Luo, M., Novackova, Z., Ordonez, S., Sramkova, Z., Holubova, M., Lysak, D., Avery, L., Caro, A. A., Crowder, R. N., Diaz-Martinez, L. A., Donley, D. W., Giorno, R. R., Reed, I. K. G., & Hensley, L. L. (2024). Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study. PLoS ONE, 19(12), 1–20. https://doi.org/10.1371/journal.pone.0314992
Publication/Presentation Information
PLoS ONE, 19(12), 2024, 1–20.