JSU Student Symposium 2022
Date
2-16-2022
Faculty Mentor
Roger Sauterer, Biology
Files
Download Full Text (438 KB)
Submission Type
Poster
Location
2:00-2:10pm | Houston Cole Library, 11th Floor
Description
Mammalian histones have been known to be one of the most highly alkaline proteins found in the nuclei. Their main function is to organize DNA into chromosomes and regulate transcription. An example of a histone would be Histone H2B, which this project focuses heavily on. Histone H2B has been seen to bind poorly to antibodies and result in a weak signal. The goal of this project is to test a multitude of blocking buffers, in triplicate, in order to find a blocking buffer that can obtain the strongest signal from the antibodies being tested. Through the use of protein gel electrophoresis and western blotting procedures, the different blocking buffers could be compared. The blocking buffers used for these experiments consisted of 5% nonfat dry milk in Tris-buffered saline (otherwise known as BLOTTO control), 2.5% Milk + 2.5% Hemoglobin, 7% Hemoglobin, 1% Milk +4 % Hemoglobin, and 5% Hemoglobin. After conducting the experiments, it was concluded that hemoglobin is not a useful blocking buffer.
Keywords
student research, biology
Rights
This content is the property of Jacksonville State University and is intended for non-commercial use. Video and images may be copied for personal use, research, teaching or any "fair use" as defined by copyright law. Users are asked to acknowledge Jacksonville State University. For more information, please contact digitalcommons@jsu.edu.
Disciplines
Biology
Recommended Citation
Al-Saadi, Noha; Mix, Grace; Thompson, Mardasia; and Zaman, Elin, "Effects of Specific Blocking Buffers on Histone H2B Antibodies" (2022). JSU Student Symposium 2022. 49.
https://digitalcommons.jsu.edu/ce_jsustudentsymp_2022/49