The Preliminary Developmental Toxicity of Methylene Blue and Orange G to Grass Shrimp Embryos
James Rayburn, Biology
3:00-3:10pm | Houston Cole Library, 11th Floor
This experiment was formed to determine the effects of Methylene Blue and Orange G on the eggs of Palaemontes pugio (Grass Shrimp). We extracted the eggs from already gravid female shrimps we acquired in the marshlands from Dauphin Island and submerged the eggs into varying levels of concentrations of Methylene Blue and Orange G. Three experiments were conducted where the eggs were separated into well plates (24 wells per plate) with each plate containing a concentration of dye. We observed the eggs hatch, the coloration of the hatchlings, and mortality rates within the span of a week per experiment. The hypothesis anticipated the higher concentrations of Orange G and Methylene Blue would be more or less toxic to the hatchlings. Our results showed that Methylene Blue was more toxic than Orange G in their respective mL concentrations. These results show the utility of using the Grass Shrimp embryos for assessing the toxicity of chemicals such as Methylene Blue and Orange G.
student research, biology
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O'Barr, Megan; Smith, Makenna; and Richards, Asel, "The Preliminary Developmental Toxicity of Methylene Blue and Orange G to Grass Shrimp Embryos" (2022). JSU Student Symposium 2022. 12.