Evaluation of Developmental Toxicity of Bupivacaine Hydrochloride Using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX)
James Rayburn, Biology
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Bupivacaine HCl is used as a long-lasting pharmaceutical local anaesthetic to anesthetize nerves in localized area such as dental surgery sites and helps reduce opioid abuse. Bupivacaine HCl is a next generation of amino-amide anesthetics such as lidocaine. There is seemingly limited analysis on this chemicals teratogenic potential. The potential teratogenic hazard of Bupivacaine HCl was evaluated using the Frog Embryo Teratogenesis Assay-Xenopus (FETAX). Early stage embryos of the South African clawed frog, Xenopus laevis, were exposed to concentrations of Bupivacaine Hydrochloride for 4 days from small cell blastula to a free living tadpole. Plastic Petri dishes of 20 embryos in 8 mls of test solution were used as our experimental unit. Four controls and 2 replicates for each concentration were used. Every 24 hours dead embryos were removed and solutions were renewed. At the end of 4 days mortality, malformations and embryo length were recorded. The teratogenic potential of Bupivacaine HCl was calculated by dividing the 96-hr LC50 / 96-hr EC50. The 96-hr LC50 was approximately 70, 96-hr EC50 was approximately 10 yielding a teratogenic potential of Bupivacaine HCl of about 7. These results indicate that Bupivacaine HCl would cause increase risk of developmental anomalies.
student research, biology
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Childress, Meredith, "Evaluation of Developmental Toxicity of Bupivacaine Hydrochloride Using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX)" (2021). JSU Student Symposium 2021. 3.