JSU Student Symposium 2021
Title
Evaluation of Developmental Toxicity of Bupivacaine Hydrochloride Using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX)
Date
2-11-2021
Faculty Mentor
James Rayburn, Biology
Loading...
Files
Download Captioning transcript (26 KB)
Submission Type
Paper
Location
Virtual
Description
Bupivacaine HCl is used as a long-lasting pharmaceutical local anaesthetic to anesthetize nerves in localized area such as dental surgery sites and helps reduce opioid abuse. Bupivacaine HCl is a next generation of amino-amide anesthetics such as lidocaine. There is seemingly limited analysis on this chemicals teratogenic potential. The potential teratogenic hazard of Bupivacaine HCl was evaluated using the Frog Embryo Teratogenesis Assay-Xenopus (FETAX). Early stage embryos of the South African clawed frog, Xenopus laevis, were exposed to concentrations of Bupivacaine Hydrochloride for 4 days from small cell blastula to a free living tadpole. Plastic Petri dishes of 20 embryos in 8 mls of test solution were used as our experimental unit. Four controls and 2 replicates for each concentration were used. Every 24 hours dead embryos were removed and solutions were renewed. At the end of 4 days mortality, malformations and embryo length were recorded. The teratogenic potential of Bupivacaine HCl was calculated by dividing the 96-hr LC50 / 96-hr EC50. The 96-hr LC50 was approximately 70, 96-hr EC50 was approximately 10 yielding a teratogenic potential of Bupivacaine HCl of about 7. These results indicate that Bupivacaine HCl would cause increase risk of developmental anomalies.
Keywords
student research, biology
Rights
This content is the property of Jacksonville State University and is intended for non-commercial use. Video and images may be copied for personal use, research, teaching or any "fair use" as defined by copyright law. Users are asked to acknowledge Jacksonville State University. For more information, please contact digitalcommons@jsu.edu.
Disciplines
Biology
Recommended Citation
Childress, Meredith, "Evaluation of Developmental Toxicity of Bupivacaine Hydrochloride Using the Frog Embryo Teratogenesis Assay: Xenopus (FETAX)" (2021). JSU Student Symposium 2021. 3.
https://digitalcommons.jsu.edu/ce_jsustudentsymp_2021/3