The Effects of Cannabidiol (CBD) Oil on the Growth and Proliferation of Callus Cells and the In Vitro Embryogenesis in Pepper, Capsicum annum, Explants
Mijitaba Hamissou, Biology
Houston Cole Library, 11th Floor | 11:00-11:10 a.m.
In plant biotechnology, a callus is defined as a mass of unorganized undifferentiated cells. This tumor-induced mass of parenchyma cells is commonly generated in plant biotechnology laboratories for use in various physiological and genetic applications. These cells of the callus tissue mimic malignant tumor cells in terms of their uncontrolled growth and proliferation. They undergo rapid and uncontrolled division. Cannabinoids are a group of closely related compounds which include cannabidiol (CBD) and the active constituents of cannabis and are known to effect the mobility, proliferation, and growth of tumor cells in humans. Because of their effects on human tumor cells, it is hypothesized that cannabinoids will interfere with plant callus induction, proliferation and growth and that cannabinoids will negatively affect in vitro plant embryogenesis. The objectives of this study are to investigate the effects of a cannabidiol oil on the growth and development of pepper, Capsicum annum, callus tissue and its effects on in vitro embryogenesis using pepper explants. The experimental procedures consisted of culturing pepper callus and pepper explants on tumor inducing media (2MS) supplemented with CBD oil media and on embryo promoting media supplemented with CBD oil. Preliminary data indicated that CBD oil caused intense oxidation of the callus tissues and that CBD oil decreased the number of in vitro embryos formation in pepper explants.
student presentations, student papers, cannabidiol, CBD
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Maxwell, Jessica, "The Effects of Cannabidiol (CBD) Oil on the Growth and Proliferation of Callus Cells and the In Vitro Embryogenesis in Pepper, Capsicum annum, Explants" (2020). JSU Student Symposium 2020. 36.