Mijitaba Hamissou, Biology
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The process of stabilizing biological materials at cryogenic temperatures is called cryopreservation. The practical example of cryobiology is the study of life at low temperatures. The use of cryopreservation is to recover the preserved living cells and tissues when brought out to ambient temperature. Advances in cryopreservation technology have led to methods that allow low temperature maintenance of a variety of tissues, cell types and subcellular materials. Low temperature preservation has been used intensively in food industry and in medicine. Cryopreservation techniques are applicable for the preservation of microorganisms, tissues, primary cells, and small multicellular organisms. The freezing process involves complex phenomena that are not fully understood. During sudden freezing, a process called vitrification transform the cytoplasmic water into sharp edge-ice crystals. The ice crystals poke holes through the cellular and organelles membranes. This causes leakage to occur at all level in the cell interior and extracellular environment causing cells and tissue death. Some microscopic organisms can survive freezing by replacing most of their internal water with low molecular weight solutes to prevent crystallization. It was shown that plant cells will respond to environmental stresses, heat, cold, by synthesizing and accumulating low-molecular-weight compounds and proteins and amino acids in their cytoplasm. These low molecular weight compounds act as cellular osmoprotectants to help protect the cell life. The increase in solute concentrations inside the cytoplasm can equally be detrimental to the cell survival. For this reason, we set our objective to investigate two possible freezing methods and insure greater chances of recovering viable cells. Tobacco, Nicotiana tobacum, cells grown in liquid Murashige and Skoog (MS) media containing 30g/L sucrose and 2 mg/L 2,4-D, and 0.1 mg/L kinetin were pretreated with different concentrations of Dimethyl sulfoxide (DMSO) and glycerol and stored at -80oC for 7 days. The cells were brought back to room temperature gradually and their viability determined. The cells were also plated on 2MS media and their growth was recorded.
student presentations, student papers, cryogenics, cryopreservation
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Cell Biology | Plant Biology
Baldwin, Alexa Grace and West, Taylor, "Possible Method of Storing Plant Cells at Subfreezing Temperatures and Recovering Viable Cells" (2020). JSU Student Symposium 2020. 16.