Department
Biology
Document Type
Article
Publication Date
11-16-2023
Abstract
Genetic analysis in model systems using bioinformatic approaches provides a rich context for a concrete and conceptual understanding of gene structure and function. With the intent to engage students in research and explore disease biology utilizing the nematode Caenorhabditis elegans model, we developed a semester-long course-based undergraduate research experience (CURE) in a hybrid (online/in-person) learning environment—the gene-editing and evolutionary nematode exploration CURE (GENE-CURE). Using a combination of bioinformatic and molecular genetic tools, students performed structure-function analysis of disease-associated variants of uncertain significance (VUS) in human orthologs. With the aid of a series of workshop-style research sessions, students worked in teams of two to six members to identify a conserved VUS locus across species and design and test a polymerase chain reaction-based assay for targeted editing of a gene in the nematode and downstream genotyping. Research session discussions, responsible conduct of research training, electronic laboratory notebook, project reports, quizzes, and group poster presentations at a research symposium were assessed for mastery of learning objectives and research progress. Self-reflectionswere collected from students to assess engagement, science identity, and science efficacy.Qualitative analysis of these reflectionsindicated several gains suggesting that all students found many aspects of the GENE-CURE rewarding (learning process of research, self-confidencein research and science identity, and personal interest) and challenging (iterative research and failure, time management, COVID-19 pandemic, and life issues).
Recommended Citation
Forte, Natalie; Veasey, Virginia; Christie, Bethany; Carter, Amira; Hanks, Marli; Holderfield, Alan; Houston, Taylor; Challa, Anil; and Turner, Ashley, "Engaging students in a genetics course-based undergraduate research experience utilizing Caenorhabditis elegans in hybrid learning to explore human disease gene variants" (2023). Research, Publications & Creative Work. 183.
https://digitalcommons.jsu.edu/fac_res/183
Included in
Biology Commons, Curriculum and Instruction Commons, Educational Assessment, Evaluation, and Research Commons, Educational Methods Commons, Genetics Commons, Molecular Genetics Commons, Research Methods in Life Sciences Commons, Scholarship of Teaching and Learning Commons, Science and Mathematics Education Commons
Publication/Presentation Information
Journal of Microbiology and Biology Education
10.1128/jmbe.00078-23