The Use of Xenopus Embryos for Determining the Developmental Toxicity of Acid Fuchsin
James Rayburn, Biology
9:00-9:10am | Houston Cole Library, 11th Floor
Acid fuchsin is a dye that is used in materials such as textile fabrics, silk, nylon, wool, and leather. These materials may pollute the water, due to synthetic dye pollution. This pollution can be harmful to the animals in these environments. Amphibians are aquatic organisms that are sensitive to harmful pollutants and there has been a large reduction of amphibian populations in the past 40 years. To determine if acid fuchsin can potentially cause developmental effects to amphibians the Frog Embryo Teratogenesis Assay -Xenopus (FETAX) was used. This assay uses early-stage frog embryos exposed for 4 days (96-hrs) in various concentrations of acid fuchsin. During this time they develop from an early-stage cell blastula to a free-living tadpole. Plastic petri dishes of embryos in 8 MLs of test solution was used as the experimental unit. Four controls and 2 replicates for each concentration were used. Every 24 hours dead embryos were removed, and solutions were renewed. At the end of the four days mortality, malformations and embryo length were recorded. Means, Standard error, probit analysis (for LC50 and EC50(malformation)), ANOVA and Bonferroni’s post hoc test were calculated using Systat. The teratogenic potential was calculated using the formula 96-hr LC50/96-hr EC50(malformation). The 96-hr LC50 was >1000 and the 96-hr EC50(malformation) was approximately 120 mg/L. At the high concentrations length was significantly affected. Also, there were severe malformations of spine, eye and gut. These preliminary results indicate that acid fuchsin may be teratogenic and further testing is needed.
student research, biology
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Fowler, Vaderick, "The Use of Xenopus Embryos for Determining the Developmental Toxicity of Acid Fuchsin" (2023). JSU Student Symposium 2023. 3.