Mijitaba Hamissou, Biology
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Cauliflower (Brassica oleracea) is a useful subject in the field of plant mitochondrial and other biochemical studies as a large part of the plant is colorless. The cells of the cauliflower can be broken open to isolate specific organelles without chlorophyll contamination. Repeatedly buying cauliflower plants introduces a large number of variables into every study. Very few reports on the culture and growth of cauliflower cells in the lab exist. This experiment seeks to develop a protocol for the lab culture and production of cauliflower cells for use in further plant studies. A liquid culture was the method chosen as many studies performed require cells rather than tissues. The protocol describes the disinfection and collection of cells from a store-bought cauliflower plant. The cells were then placed into the liquid culture based on Murashige and Skoog’s growth and culture media. The media also contains gibberellic acid to induce the growth of the growth of the apical meristem cells of the cauliflower (otherwise known as the “curd”), pre-mixed W5 solution to produce protoplasts of the plant tissue, as well as an auxin. Three auxins of varying concentrations were tested for efficacy— 3-indoleacetic acid (IAA), indole-3-butyric acid (IBA), and 1-naphthaleneacetic acid (NAA). The cell cultures were then incubated on a rotary shaker at 27 C and periodically sampled under a microscope to quantify health and growth. Visual tests were then performed on the protoplasts using vitality stains, as well as biochemical tests to further examine protoplast viability for other experiments.
student presentations, student posters, cauliflower
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Watral, Kyra and Thompson, Diana, "Production of Competent Cauliflower Protoplast" (2020). JSU Student Symposium 2020. 18.